Oxidative Stress Induced by Diclofenac Alone and under the Influence of Certain Variables in Broilers

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Authors

  • C. Haritha
     Departments of Pharmacology and Toxicology, and College of Veterinary Science, Rajendranagar, Hyderabad - 500 030 ,IN
  • A. Gopala Reddy
     Departments of Pharmacology and Toxicology, and College of Veterinary Science, Rajendranagar, Hyderabad - 500 030 ,IN
  • Y. Anjaneyulu
     Veterinary Pathology, College of Veterinary Science, Rajendranagar, Hyderabad - 500 030 ,IN
  • B. Kalakumar
     Departments of Pharmacology and Toxicology, and College of Veterinary Science, Rajendranagar, Hyderabad - 500 030 ,IN
  • G. Dilip Reddy
     Departments of Pharmacology and Toxicology, and College of Veterinary Science, Rajendranagar, Hyderabad - 500 030 ,IN

Keywords:

Diclofenac, hepatotoxicity, high-protein high-calcium diet, oxidative stress
Virology

Abstract

Diclofenac toxicity was studied in male broiler chicks (Cobb strain) of a day old age. The chicks were randomly divided into eight groups consisting of 10 in each group. Group 1 was kept as basal diet control (1–32 days), group 2 on basal diet for 32 days + diclofenac (0.8 mg/kg body weight I/M) on day 24, 26, 28, 30, and 32, group 3 on basal diet for 32 days + cyclophosphamide (50 mg/kg body weight I/M once daily) from day 20 to 23, group 4 on high-protein, high-calcium, low vitamin A (HPHC) diet (1–32 days), group 5 on basal diet + diclofenac + cyclophosphamide (as per the schedule), group 6 on HPHC + diclofenac (as per the schedule), group 7 on HPHC + cyclophosphamide (as per the schedule), and group 8 on HPHC + diclofenac + cyclophosphamide (as per the schedule). The activity of TBARS, SOD, and catalase revealed a significant (P <0.05) increase, while there was a significant (P < 0.05) decrease in the concentration of GSH in liver in the groups given diclofenac either alone or in combination with other variables. There was no significant difference in the diclofenac residue levels. The electron microscopy of liver revealed lesions of mild to marked severity in different combinations. It can be concluded that diclofenac has the toxic potential in poultry at subtherapeutic doses and further the toxic effects were more pronounced under the influence of immunosuppressants and HPHC diet.

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Published

2018-05-15

How to Cite

Haritha, C., Reddy, A. G., Anjaneyulu, Y., Kalakumar, B., & Reddy, G. D. (2018). Oxidative Stress Induced by Diclofenac Alone and under the Influence of Certain Variables in Broilers. Toxicology International, 17(1), 27–29. Retrieved from https://informaticsjournals.com/index.php/toxi/article/view/21162

Issue

Volume 17, Issue 1, January-June 2010

Section

Original Research
Received 2018-05-14
Accepted 2018-05-14
Published 2018-05-15

 

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