Toxicology International

Clinicopathological Studies on Vitamin D3 Toxicity and Therapeutic Evaluation of Aloe vera in Rats


Affiliations

  • GADVASU, Departments of Veterinary Pathology, Ludhiana, Punjab, 141 004, India
  • GADVASU, Departments of Veterinary Pathology,, Ludhiana, Punjab, 141 004, India
  • GADVASU, Departments of Veterinary Pharmacology and Toxicology, Ludhiana, Punjab, 141 004, India

Abstract

A study was conducted to examine the clinical signs, hematological, biochemical and histopathological changes in vitamin D3 toxicity at a dose rate 2 mg/kg b.wt. of vitamin D3 and to assess the protective effect of Aloe vera in vitamin D3 toxicity. The clinical signs observed were anorexia, progressive weight loss, difficulty in movement and respiration, diarrhea, epistaxis, subnormal body temperature and nervous signs before death. Mortality was observed in treated rats between day 10 and day 19 of treatment. The gross postmortem changes observed were severe emaciation, white chalky deposits on epicardial surface of heart, pin point white deposits on cortical surface of kidneys with pale yellow discoloration and diffused white deposits on serosal surface of stomach and intestine with bloody ingesta in lumen. The hematological changes included non-significant increase in hemoglobin and total leukocyte count and significant increase in relative neutrophil count. The biochemical changes observed were significant increase in plasma concentration of calcium, phosphorus and blood urea nitrogen, whereas a significant decrease in the concentration of albumin and total plasma protein was observed. The histopathological lesions included calcification of various organs, viz., tongue, stomach, intestines, kidney, heart, aorta, larynx, trachea, lungs, spleen, choroid plexus arteries of brain and vas deferens. The Aloe vera juice (2.5% in drinking water) has no protective effect on vitamin D3 toxicity (2 mg/kg b.wt.).

Keywords

Aloe vera, calcification, histopathological lesions, hypercalcemia, vitamin D3 toxicity

Subject Discipline

Botany

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References

Rumbeiha WK. Specific toxicants: Cholecalciferol. In: Peterson ME, Talcott PA, editors. Small Animal Toxicology. St. Louis, Missouri, USA: Saunders Elsevier; 2006. p. 629-42.

Luna LG. Manual of histological staining methods of Armed Forces Institute of Pathology. 3rd ed. New York: McGraw Hill Book Company; 1968.

Roberson RJ, Swecker WS, Hullender LL. Hypercalcemia and hypervitaminosis D in two lambs. J Am Vet Med Assoc 2000;216:1115-8.

Sandhu HS, Brar, RS. Textbook of Veterinary Toxicology. 2nd ed. Ludhiyana: Kalyani Publishers; 2009. p. 245.

Morrow C. Cholecalciferol poisoning. Vet Med 2001;12:905-11.

Price PA, Buckley JR, William MK. The amino bisphosphonate ibandronate prevents vitamin D induced calcification of arteries, cartilage, lungs and kidneys in rats. J Nutrition 2001;131:2910-5.

Morita T, Awakura T, Shimada A, Umemura T, Nagai T, Haruna A. Vitamin D toxicosis in cats: Natural outbreak and experimental study. J Vet Med Sci 1995;57:831-7.

Long GG. Acute toxicosis in swine associated with excessive dietary intake of vitamin D. J Am Vet Med Assoc 1984;184:164-70.

Hilbe M, Sydler T, Fischler L, Naegli H. Metastatic calcification in dog attributable to ingestion of a tacalcitol ointment. Vet Pathol 2000;37:490-2.

Kawashima H, Kurozumi S, Hashimoto Y. Aspirin inhibition of 1 alpha-hydroxyvitamin D3 or parathyroid hormone induced hypercalcemia in vivo in rats. A mechanism independent of prostaglandin biosynthesis inhibition. Biochem Pharmacol 1985;34:1901-6.

Heggers J, Robson M. Experimental and clinical observation on frostbite. Ann Emerg Med 1987;16:1056-62.

Gupta R, Flora SJ. Protective value of Aloe vera against some toxic effects of arsenic in rats. Phytother Res 2005;19:23-8.

Etim OE, Farombi EO, Usoh IF, Akpan EJ. The protective effects of Aloe vera juice on lindane induced hepatotoxicity and genotoxicity. Pak J Pharm Sci 2006;19:337-40.


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