Effects of corn oil as a vehicle- on liver of female Swiss albino mice Mus musculus

Authors

DOI:

https://doi.org/10.18311/jeoh/2010/18342

Keywords:

Corn oil, Vehicle, Liver, Biochemical, Histopathological

Abstract

The study was conducted to evaluate corn oil as a vehicle for delivery of toxins and to observe its effect on liver of female Swiss albino mice. Mice were randomly divided into five groups of six mice each and were administered corn oil at a dose of 10 ml /kg body weight orally for a period of six weeks. Mice of control Gr-1 were kept on normal diet and tap water ad libitum. Histopathological examination of the liver of female albino mice revealed mild alteration in Gr.-4 and mild fatty degeneration was observed in Gr.-5. Mice in Gr.-2 and Gr.-3 showed neither abnormal conditions nor any change in the liver architecture. The biochemical parameters like SGPT, ALP, total protein, and albumin (LFT) were estimated for all the treatment groups and compared with their normal values of the contnDl Gr.-1. Statistical analysis was done using one way analysis of variance (ANOVA). The values obtained were not significant (P>0.05) in all the treated groups for all the biochemical parameters. However the levels of ALP and Albumin in Gr.-5 were higher as compared to the control Gr-1. These data indicate that corn oil at the dose of 10 ml/kg body weight can be used as a vehicle for toxicological studies but may cause adverse effects on the hepatic tissue if used for prolonged periods, suggesting that corn oil as a vehicle can be a confounding factor in the toxicity study depending on time and dose

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Author Biographies

Tanuja .

B.M.D. College, Dayalpur, Vaishali, Bihar, India

Anjali Singh

Mahavir Cancer Sansthan, Phulwarisarif, Patna, Bihar, India

J. K. Singh

Mahavir Cancer Sansthan, Phulwarisarif, Patna, Bihar, India

A. Nath

Mahavir Cancer Sansthan, Phulwarisarif, Patna, Bihar, India

Ravish Kumar

B.M.D. College, Dayalpur, Vaishali, Bihar, India

References

Balistreri, W.F., and. Shaw, L.M. (1987) Liver Function. In: Fundamentals of Clinical Chemistry. Tietz, N.W.(ed). 3 ed., W.rd B. Saunders Company, Philadelphia, 729.

Condie, L. W., Laurie, R. D., Mills, T, Robinson, M., and Bercz, J. P. (1986) Effect of gavage vehicle on hepatotoxicity of carbon tetrachloride in CD-I mice; corn oil versus Tween-60 aqueous emulsion. Fundam. Appl. Toxicol. ,7, 199 -206.

Doumas. B.T., Watson. W.A., and Biggs, H.G. (1971) Albumin standards and the measurement of serum albumin with bromocresol green. Clin. Chim. Acta. , 31, 87-96.

Dupont, J., White, P. J., Carpenter, M. P., Schaefer, E. J., Meydani, S. N., Elson. C. E., Woods, M., and Gorbach, S. L.(1990) Food uses and health effects of corn oil. J. Am. Coll. Nutr., 9, 438 - 470.

Eaton, D. L., and Klaassen, C. D. (1995) Principles of toxicology. In Casarett and Doulfs Toxicology: The Basic Science of Poisons (C. D. Klaassen, Ed.), McGraw-Hill, New York.Sth ed. 13-33.

Edem.D.0.(2009) Haematological and histological alterations induced in rats' by palm oil containing diets. European J. Scientific Res. 32, 405-418.

Farooqui, M. Y, Ybarra, B., Piper, J., and Tamez, A. (1995) Effect of dosing vehicle on the toxicity and metabolism of unsaturated aliphatic nitriles. J. Appl. Toxicol., 15, 411-420.

Fashakin.J.B. and Unokiwedi,C.C.(1993) National evaluation of warankasi and waragusi prepared from cow milk partially substituted with melon m'llk.Nigerian Food J., 11, 128-134.

Friedman, S.F, Martin, P. and Munoz, J.S. (2003) Laboratory evaluation of the patient with liver disease. Hepatology, a text book of liver disease. PhJiedelphia; Saunders publication, 1, 661-709.

lgwebuike,J.U.,Anugwa, F.O.I., Raji, A.O., Ehioba, N.G., and Ikuriiz, S.A. (2008) Nutrient digestibility, hematological and serum biochemical indices of rabbits fed graded levels of Acacia albida pods. ARPN J. Agii. Bio. Sci., 3,33-40.

Kaneko, J.J., Harvey, J.W., and Bruss, M.L. (1997) Clinical Biochemistry of Domestic Animals. 5th edn., New York, Academic Press, 895-899.

Kaplan, M.M. (1986) Serum alkaline phosphataseanother piece is added the puzzle. Hepat, 6,526531.

Kind, P.R.N, and King, E.J. (1954) Estimation of plasma phophatase by determination of hydrolyzed phenol with amino antiryrine. J. Clin. Pathol., 7, 332-330 .

Manorama, R. and Rukmini, C. (1991) Nutritional evaluation of crude palm oil in rats. Am. J. Clin. Nutr, 53, 1031 S-1033 S.

Reitman, S. and S. Frankel.(1957) In vitro determination of transaminase activity in serum. Am. J. Clin. Pathol., 28, 56-59.

Rosaiki, S.B. and Mcintyre, N. (1999) Biochemical investigations in the management of liver disease. Oxford textbook of clinical hepatology, 2â„¢* ed. NewYork, Oxford University press, 503-521.

Rosen, H.R. and Keefe, E.B. (2000) Evaluation of abnormal liver enzymes, use of liver test and the serology of viral hepatitis : Liver diseasease diagnosis and management. 1" ed. New York; Churchill living stone publisher, 24-35.

Sherlock, S., (1997). Assessment of liver function disease of liver and biliary system, iOth ed, London; Blackwell science Ltd, 17-32.

Thapa, B.R. and Walia, A., (2007). Liver function test and their interpretation, Indian J Pediatrics, 74, 663671.

Withey, J. R., Collins, B. T. and Collins, P G. (1983) Effect of vehicle on the pharmacokinetics and uptake of four halogenated hydrocarbons from the gastrointestinal tract of the rat. J. Appl. Toxicol., 3, 249-253.

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Published

2017-12-06

How to Cite

., T., Singh, A., Singh, J. K., Nath, A., & Kumar, R. (2017). Effects of corn oil as a vehicle- on liver of female Swiss albino mice Mus musculus. Journal of Ecophysiology and Occupational Health, 10(3-4), 203–209. https://doi.org/10.18311/jeoh/2010/18342

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