Comparative Phytochemical Analysis and Free Radical Scavenging Activity of Different Extracts of Allium ascalonicum (Shallots)

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  • Department of Zoology, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore, Tamil Nadu ,IN
  • Department of Zoology, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore, Tamil Nadu ,IN
  • Department of Zoology, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore, Tamil Nadu



Allium ascalonicum, Phytochemical Screening, Free Radical Scavenging Activity.


Allium ascalonicum belongs to family Alliaceae, is an annual plant spread throughout India. It is commonly known as shallots in India. The screening of various phytochemicals present in the methanol, ethanol, chloroform and water extracts of Allium ascalonicum was performed using a standard procedure. The different extracts were measured for the radical scavenging activities in vitro against a battery of radicals namely DPPH, FRAP and H2O2 and also the reducing power potential. The phytochemical analysis led to the detection of alkaloids, flavonoids, steroids, phenols, amino acids, glycosides, tannins, triterpenoids and carbohydrates. Of all the extracts, maximum intensity was revealed in the methanol extract. Similarly, the methanol extract exhibited a stupendous radical scavenging effect with respect to all the radicals in a dosage dependent manner where the scavenging capacity increased with increase in concentration. The DPPH, FRAP and H2O2 showed a maximum inhibition, 80.67, 77.12 and 80.88 % respectively at 40 μg/mlas compared to the standard, 87.38%. Likewise, the reducing power ability was shown in the dose dependent manner. This study provides evidence for the presence of one or more soluble constituents in the plants used, that probably interferes with radical scavenging effect and further could be opted for other therapeutic activities.


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How to Cite

V. M. H., Z., E., D., & K. S., S. (2021). Comparative Phytochemical Analysis and Free Radical Scavenging Activity of Different Extracts of <i>Allium ascalonicum</i> (Shallots). The Indian Journal of Nutrition and Dietetics, 58(S2), 58–67.



Original Articles



Verpoorte, R. Chemodiversity and the biological role of secondary metabolites, some thoughts for selecting plant material for drug development. In Bioassay Methods in Natural Product Research and Drug Development, 1999, 11-23. Springer, Dordrecht. DOI:

Qi, Z. Who traditional medicine strategy, 2013, 2014-2023. Geneva: World Health Organization.

Edeoga, H.O., Okwu, D.E. and Mbaebie, B.O. Phytochemical constituents of some Nigerian medicinal plants. Afr. J. Biotechnol., 2005, 4, 685-688. DOI:

Tumwine, J. Implementation of the framework convention on tobacco control in Africa: Current status of legislation. Int. J. Environ. Res. Pub. Heal., 2011, 8, 4312-4331. DOI:

Goud, J.V., Suryam, A. and Charya, M.S. Biomolecular and phytochemical analyses of three aquatic angiosperms. Afr. J. Microbial. Res., 2009, 3, 418-421. DOI:

Pourmorad, F., Hosseinimehr, S.J. and Shahabimajd, N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr. J. Biotechnol., 2006, 5, 1142-1145.

Duh, P.D., Tu, Y.Y. and Yen, G.C. Antioxidant activity of water extract of HarngJyur (Chrysanthemum morifolium Ramat). LWT-Fd. Sci. Technol., 1999, 32, 269-277. DOI:

Molyneux, P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol., 2004, 26, 211-219.

Adeniyi, B.A. and Anyiam, F. M. In vitro anti-Helicobacter pylori potential of methanol extract of Allium ascalonicum Linn. (Liliaceae) leaf: susceptibility and effect on urease activity. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 2004, 18, 358-361. DOI:

Dankert, J., Tromp, T.F., De Vries, H. and Klasen, H.J. Antimicrobial activity of crude juices of Allium ascalonicum, Allium cepa and Allium sativum. Zentralblatt fur Bakteriologie, Originale. A: 1979, 245, 229.

Amin, M. and Kapadnis, B.P. Heat stable antimicrobial activity of Allium ascalonicum against bacteria and fungi. Ind. J. Experim. Biol. 2005, 43, 751-754.

Tappayuthpijarn, P., Dejatiwongse, Q., Hincheranan, T. and Suriyant, P.N. Effect of Allium ascalonicum on erythrocyte shape in induced hypercholesterolemia rabbits. J. Med. Assoc. Thailand- Chotmaihetthangphaet, 1989, 72, 448.

Kokate, C.K., Purohit, A.P. and Gokhale, S.B. Pathway to screen phytochemical nature of natural drugs. Pharmacognosy. 39th ed. India, NiraliPrakashan, 2007, 607-611.

Mensor, L.L., Menezes, F. S., Leití£o, G.G., Reis, A.S., Santos, T.C.D., Coube, C.S. and Leití£o, S.G. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother. Res., 2001, 15, 127-130. DOI:

Dehghan, G. and Khoshkam, Z. Tin (II)–quercetin complex: Synthesis, spectral characterisation and antioxidant activity. Fd. Chem., 2012, 131, 422-426. DOI:

Ruch, R.J., Cheng, S.J. and Klaunig, J.E. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogen., 1989, 10, 1003-1008. DOI:

Oyaizu, M. Studies on product of browning reaction prepared from glucosamine. Jap. J. Nutr., 1986, 44, 307-315. DOI:

Vanitha, T., Sumathy, H., Sangeetha, J., Devaki, B. and Vijayalakshmi, K. Phytochemical analysis of Allium ascalonicum. Biomed., 2009, 29, 22-25.

Vanitha, T., Umadevi, K.J. and Vijayalakshmi, K. Free radical scavenging activity of Allium ascalonicum. The Bioscan., 2011, 6, 215-218.

Koleva, I.I., Van Beek, T.A., Linssen, J.P.H., De Groot, A. and Evstatieva, L.N. Screening of plant extracts for antioxidant activity- A comparative study on three testing methods. Phytochem. Analy.. 2002, 13, 8-17. DOI:

Kessler, M., Ubeaud, G. and Jung, L. Anti and pro-oxidant activity of rutin and quercetin derivatives. J. Pharma. Pharmacol., 2003, 55, 131-142. DOI:

Connor, A.M., Luby, J.J., Hancock, J.F., Berkheimer, S. and Hanson, E.J. Changes in fruit antioxidant activity among blueberry cultivars during cold-temperature storage. J. Agricul. Fd. Chem., 2002, 50, 893-898. DOI:

Kaur, C. and Kapoor, H.C. Anti oxidant activity and total phenolic content of some Asian vegetables. Int. J. Fd. Sci. Technol., 2002, 37, 153-161. DOI:

Moyer, R.A., Hummer, K.E., Finn, C.E., Frei, B. and Wrolstad, R.E. Anthocyanins, phenolics and antioxidant capacity in diverse small fruits: Vaccinium, Rubus and Ribes. J. Agricul. Fd. Chem., 2002, 50, 519-525. DOI:

Miller, H.E., Rigelhof, F., Marquart, L., Prakash, A. and Kanter, M. Antioxidant content of whole grain breakfast cereals, fruits and vegetables. J. Am. Colle. Nutr., 2000, 19, 312-319. DOI:

Heinonen, I.M. and Meyer, A.S. Antioxidants in fruits, berries and vegetables. Fruit and vegetable processing (W Jongen, ed.), Woodhead Publishing Limited, Cambridge, 2002, 23-51. DOI:

Gennaro, L., Leonardi, C., Esposito, F., Salucci, M., Maiani, G., Quaglia, G. and Fogliano, V. Flavonoid and carbohydrate contents in Tropea red onions: Effects of homelike peeling and storage. J. Agricul. Fd. Chem., 2002, 50, 1904-1910. DOI: