Lead-induced Hepatotoxicity and Evaluation of Certain Anti-stress Adaptogens in Poultry


  • College of Veterinary Science, Rajendranagar, Departments of Pharmacology and Toxicology, Hyderabad, 500 030, India
  • College of Veterinary Science, Rajendranagar, Hyderabad, Departments of Pharmacology and Toxicology, 500 030, India
  • College of Veterinary Science, Korutla, Andhra Pradesh, India
  • College of Veterinary Science, Rajendranagar, Department of Pathology, Hyderabad, 500 030, India


A total of 225 day-old sexed male broiler chicks (Vencobb strain) were divided randomly into 15 groups consisting of 15 chicks in each group to study the toxicity of lead on hepatocytes. Group 1 was maintained on basal diet, group 2 on polyherbal formulation (PHF; stressroak), group 3 on shilajith, group 4 on amla and group 5 on vit E + Se. Group 6 was maintained on lead for 6 weeks and group 7 on lead for 4 weeks and subsequently on basal diet without lead for the remaining 2 weeks. Groups 8, 9, 10 and 11 were given lead along with PHF, shilajith, amla and vit E + Se, respectively, throughout 6 weeks. Groups 12, 13, 14 and 15 were given lead containing diet for the first 4 weeks and subsequently treated with PHF, shilajith, amla and vit E + Se, respectively, for the remaining 2 weeks. The activity of alanine transaminase (ALT) was significantly (P<0.05) increased in the toxic control groups at the end of 4th week as compared to group 1. However, following treatment, there was a significant (P<0.05) reversal in groups 12–15. The activity of Na+/K+-ATPase, Ca2+ATPase, Mg2+ATPase and CYP450 was significantly (P<0.05) reduced in the liver of toxic control groups 6 and 7 as compared to groups1 through 5, which had the maximum activity of all the groups. Groups 8 through 15 revealed a significant (P<0.05) increase in the activity of these hepatocytic enzymes. The histological sections of the liver in lead toxic control (group 6) showed moderate focal lymphoid aggregates in liver, whereas the lesions were mild to moderate in treated groups and there were no observable lesions in plain control groups. The study revealed protective effect of PHF (stressroak), shilajith, amla and vit E + Se in lead-induced hepatocytic damage.


ATPase, broilers, CYP450, lead, hepatotoxicity

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Flora SJ, Dube SN. Chelating agents for the treatment of lead intoxication. Indian J Environ Toxicol 1995;5:1-6.

Gurer H, Ercal N. Can antioxidants be beneficial in the treatment of lead poisoning? Free Radical Biol Med 2000;29:927-45.

Bakalli RI, Pesti GM, Ragland WL. The magnitude of lead toxicity in broiler chickens. Vet Human Toxicol 1995;37:15-9.

Suketa Y, Ujiie M, Okada S. Alteration of sodium and potassium mobilization and of adrenal function by long-term ingestion of lead. Biochem Pharmacol 1982;31:2913-9.

Yallapragada PR, Butler J, Kumar BK, Rajanna B. In vitro effect of lead on Na+, K+-ATPase activity in different regions of adult rat brain. Drug Chem Toxicol 2003;26:117-24.

Upasani CD, Balaraman R. Effect of vitamin E, vitamin C and spirulina on the levels of membrane bound enzymes and lipids in some organs of rats exposed to lead. Indian J Pharmacol 2001;33:185-91.

Bopanna KN, Balaraman R, Nadig RS. Antioxidant status of s-allyl cysteine sulphoxide on monosodium glutamate potentiated atherogenesis. Indian J Pharmacol 1998;30:73-81.

Sadasivudu B, Indira Rao T, Murthy CR. Acute metabolic effects of ammonia in mouse brain. Neurochem Res 1977;2:639-55.

Kodama T, Fukui K, Kometani K. The initial phosphate burst in ATP hydrolysis by myosin and subfragment-1 as studied by a modified malachite green method for determination of inorganic phosphate. J Biochem 1986;99:1465-72.

Cinti DL, Moldeus P, Schenkman JB. Kinetic parameters of drugmetabolizing enzymes in Ca+2-sedimented microsomes from rat liver. Biochem Pharmacol 1972;21:3249-56.

Adav SS, Padmawar PA, Govindwar SP. Effect of sodium sulfadimethylpyrimidine on multiple forms of cytochrome P450 in chicken. Indian J Pharmacol 2005;37:169-73.

Lowry OH, Rosenbrough MJ, Farr AL, Randell RA. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-75.

Omura T, Sato R. The carbon monoxide-binding pigment of liver microsomes. J Biol Chem 1964;239:2370-8.

Kaneko JJ, Harvey JW, Michael, LB. Clinical Biochemistry of Domestic Animals. 5th ed. New York: Academic press; 1997.

Pramyothin P, Samosorn P, Poungshompoo S, Chaichantipyuth C. The protective effects of Phyllanthus emblica Linn. extract on ethanol induced rat hepatic injury. J Ethnopharmacol 2006;107:361-4.

Knowles SO, Donaldson WE. Dietary lead alters fatty acid composition and membrane peroxidation in chick liver microsomes. Poult Sci 1996;75:1498-500.


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